Once you have uploaded your keystore file and provided your keystore credentials on the Code Signing tab of the App Settings page, the Android Sign Step signs your APK digitally. Bitrise assigns Environment Variables to the uploaded file and credentials, and uses those in the respective fields of the Android Sign Step. Once the Step runs, it produces a signed APK or App Bundle which will be used as the input value of the App file path field in the Google Play Deploy Step.
Make sure you have the Android Sign Step right after a build Steps but before Deploy to Google Play Step in your deploy workflow. If you wish to get your Android project signed automatically, use the Android Sign Step and do not set any gradle task for the signing, otherwise, the Step will fail.